Although used in academicresearch for several decades, 3D culture models have long been regarded expensive, cumbersome and unnecessary in drug development processes.
Technical advances, coupled with recent observations showing that gene expression in 3D is much closer to clinical expression profiles than those seen in 2D, have renewed attention and generated hope in the feasibility of maturing organotypic 3D systems to therapy test platforms with greater power to predict clinical efficacies.
Here we describe a standardized setup for reproducible, easy-handling culture, treatment and routine analysis of multicellular spheroids, the classical 3D culture system resembling many aspects of the pathophysiological situation in human tumor tissue.
We discuss essential conceptual and practical considerations for an adequate establishment and use of spheroid-based drug screening platforms and also provide a list of human carcinoma cell lines, partly on the basis of the NCI-DTP 60-cell line screen, that produce treatable spheroids under identical culture conditions.
In contrast to many other settings with which to achieve similar results, the protocol is particularly useful to be integrated into standardized large-scale drug test routines as it requires a minimum number of defined spheroids and a limited amount of drug.
The estimated time to run the complete screening protocol described herein–including spheroid initiation, drug treatment and determination of the analytical end points (spheroid integrity, and cell survival through the acid phosphatase assay)–is about 170 h. Monitoring of spheroid growth kinetics to determine growth delay and regrowth, respectively, after drug treatment requires long-term culturing >> or =14 d).
KT 5823 |
B6673-.1 |
ApexBio |
100 µg |
EUR 112 |
|
Description: protein kinase G inhibitor |
KT 5823 |
B6673-.25 |
ApexBio |
250 µg |
EUR 249 |
|
Description: protein kinase G inhibitor |
KT 5720 |
B9002-.05 |
ApexBio |
50 µg |
EUR 77 |
|
Description: protein kinase A inhibitor, potent and selective |
KT 5720 |
B9002-.1 |
ApexBio |
100 µg |
EUR 146 |
|
Description: protein kinase A inhibitor, potent and selective |
KT 5720 |
B9002-.25 |
ApexBio |
250 µg |
EUR 345 |
|
Description: protein kinase A inhibitor, potent and selective |
KT 5720 |
B9002-.5 |
ApexBio |
500 µg |
EUR 611 |
|
Description: protein kinase A inhibitor, potent and selective |
KT 5823 |
20-abx076629 |
Abbexa |
-
Ask for price
-
Ask for price
|
|
|
KT 5823 |
SIH-458-100UG |
Stressmarq |
100 µg |
EUR 141 |
Description: PKG kinase inhibitor |
KT 5823 |
SIH-458-500UG |
Stressmarq |
500 µg |
EUR 505 |
Description: PKG kinase inhibitor |
KT-5720 |
abx076628-250l |
Abbexa |
250 µl |
EUR 212.5 |
KT-5720 |
abx076628-500l |
Abbexa |
500 µl |
EUR 487.5 |
KT-5823 |
abx076629-250l |
Abbexa |
250 µl |
EUR 168.75 |
KT-5823 |
abx076629-500l |
Abbexa |
500 µl |
EUR 462.5 |
KT-362 fumarate |
T27753-10mg |
TargetMol Chemicals |
10mg |
Ask for price |
|
Description: KT-362 fumarate |
KT-362 fumarate |
T27753-50mg |
TargetMol Chemicals |
50mg |
Ask for price |
|
Description: KT-362 fumarate |
Forchlorfenuron (KT-30, CPPU) |
C7187-1000 |
ApexBio |
1g |
EUR 68 |
|
Forchlorfenuron (KT-30, CPPU) |
C7187-500 |
ApexBio |
500mg |
EUR 40 |
|
Ketoconazole KT 10 mcg |
SD224-1PK |
EWC Diagnostics |
1 unit |
EUR 6.31 |
Description: Ketoconazole KT 10 mcg |
Ketoconazole KT 10 mcg |
SD224-1VL |
EWC Diagnostics |
1 unit |
EUR 2.99 |
Description: Ketoconazole KT 10 mcg |
Ketoconazole KT 10 mcg |
SD224-5CT |
EWC Diagnostics |
1 unit |
EUR 6.65 |
Description: Ketoconazole KT 10 mcg |
Ketoconazole KT 10 mcg |
SD224-5VL |
EWC Diagnostics |
1 unit |
EUR 11.84 |
Description: Ketoconazole KT 10 mcg |
Ketoconazole KT 10 mcg |
SD224-5X50DS |
EWC Diagnostics |
1 unit |
EUR 6.5 |
Description: Ketoconazole KT 10 mcg |
Ketoconazole KT 50 mcg |
SD274-1PK |
EWC Diagnostics |
1 unit |
EUR 6.31 |
Description: Ketoconazole KT 50 mcg |
Ketoconazole KT 50 mcg |
SD274-1VL |
EWC Diagnostics |
1 unit |
EUR 2.99 |
Description: Ketoconazole KT 50 mcg |
Ketoconazole KT 50 mcg |
SD274-5CT |
EWC Diagnostics |
1 unit |
EUR 6.65 |
Description: Ketoconazole KT 50 mcg |
Ketoconazole KT 50 mcg |
SD274-5VL |
EWC Diagnostics |
1 unit |
EUR 11.84 |
Description: Ketoconazole KT 50 mcg |
Ketoconazole KT 50 mcg |
SD274-5X50DS |
EWC Diagnostics |
1 unit |
EUR 6.5 |
Description: Ketoconazole KT 50 mcg |
Ketoconazole KT 30 mcg |
SD275-1PK |
EWC Diagnostics |
1 unit |
EUR 6.31 |
Description: Ketoconazole KT 30 mcg |
Ketoconazole KT 30 mcg |
SD275-1VL |
EWC Diagnostics |
1 unit |
EUR 2.99 |
Description: Ketoconazole KT 30 mcg |
Ketoconazole KT 30 mcg |
SD275-5CT |
EWC Diagnostics |
1 unit |
EUR 6.65 |
Description: Ketoconazole KT 30 mcg |
Ketoconazole KT 30 mcg |
SD275-5VL |
EWC Diagnostics |
1 unit |
EUR 11.84 |
Description: Ketoconazole KT 30 mcg |
Ketoconazole KT 30 mcg |
SD275-5X50DS |
EWC Diagnostics |
1 unit |
EUR 6.5 |
Description: Ketoconazole KT 30 mcg |
Plasminogen Receptor (KT) (PLGRKT) Antibody |
20-abx318476 |
Abbexa |
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
-
Ask for price
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Plasminogen Receptor (KT) (PLGRKT) Antibody |
abx318476-100l |
Abbexa |
100 µl |
EUR 250 |
Plasminogen Receptor (KT) (PLGRKT) Antibody |
abx318476-50l |
Abbexa |
50 µl |
EUR 162.5 |
11-KT( 11-keto Testosterone) ELISA Kit |
ELK8908-48T |
ELK Biotech |
48T |
Ask for price |
|
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 11-KT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 11-KT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-KT in the samples is then determined by comparing the OD of the samples to the standard curve. |
11-KT( 11-keto Testosterone) ELISA Kit |
ELK8908-96T |
ELK Biotech |
96T |
Ask for price |
|
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 11-KT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 11-KT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-KT in the samples is then determined by comparing the OD of the samples to the standard curve. |
Fish 11-keto Testosterone (KT) ELISA Kit |
EK11227 |
SAB |
96Т |
EUR 799 |
|
Fish 11-keto Testosterone (KT) ELISA Kit |
AE58425FI-48Tests |
Abebio |
48 Tests |
EUR 290 |
|
Description: Fish |
Fish 11-keto Testosterone (KT) ELISA Kit |
AE58425FI-96Tests |
Abebio |
96 Tests |
EUR 540 |
|
Description: Fish |
Human 11-Ketotestosterone, 11-KT GENLISA ELISA |
KBH5106 |
Krishgen |
1 x 96 wells |
EUR 286 |
Human11-keto Testosterone (11-KT) ELISA Kit |
NSL2276Hu |
Sunlong |
96 wells |
EUR 468 |
|
B30C-4000-483OR-KT AF Systems Consumables |
139928 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483OR-KT AF Systems Consumables |
139929 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483OR-KT AF Systems Consumables |
139930 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-4000-483BK-KT AF Systems Consumables |
139931 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483BK-KT AF Systems Consumables |
139932 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483BK-KT AF Systems Consumables |
139933 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-4000-483GN-KT AF Systems Consumables |
139934 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483GN-KT AF Systems Consumables |
139935 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483GN-KT AF Systems Consumables |
139936 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-4000-483BL-KT AF Systems Consumables |
139937 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483BL-KT AF Systems Consumables |
139938 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483BL-KT AF Systems Consumables |
139939 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-4000-483RD-KT AF Systems Consumables |
139940 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483RD-KT AF Systems Consumables |
139941 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483RD-KT AF Systems Consumables |
139942 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-4000-483YL-KT AF Systems Consumables |
139943 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-3000-483YL-KT AF Systems Consumables |
139944 |
BRADY NV |
Box of 1 Roll |
EUR 473.22 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483YL-KT AF Systems Consumables |
139945 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483YL-KT AF Systems Consumables |
139946 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-4000-483WT-KT AF Systems Consumables |
139947 |
BRADY NV |
Box of 1 Roll |
EUR 563.88 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-3000-483WT-KT AF Systems Consumables |
139948 |
BRADY NV |
Box of 1 Roll |
EUR 473.22 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-2250-483WT-KT AF Systems Consumables |
139949 |
BRADY NV |
Box of 1 Roll |
EUR 415.4 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
B30C-1125-483WT-KT AF Systems Consumables |
139950 |
BRADY NV |
Box of 1 Roll |
EUR 274.35 |
Description: Description Dutch: ToughStripe-vloertape voor BBP35/BBP37/S3xxx/i3300-printers; Description French: Ruban de marquage au sol ToughStripe pour imprimantes BBP35/BBP37/S3xxx/i3300 |
Human Plasminogen Receptor (KT) (PLGRKT) ELISA Kit |
abx539314-96tests |
Abbexa |
96 tests |
EUR 687.5 |
Mouse Plasminogen Receptor (KT) (PLGRKT) ELISA Kit |
abx539315-96tests |
Abbexa |
96 tests |
EUR 687.5 |
Rat Plasminogen Receptor (KT) (PLGRKT) ELISA Kit |
abx539316-96tests |
Abbexa |
96 tests |
EUR 687.5 |
Rat Chondroitin Sulfate Epitope 3B3(CS3B3 ELISA Kt |
GA-E0451RT-48T |
GenAsia Biotech |
48T |
EUR 380.4 |
Rat Chondroitin Sulfate Epitope 3B3(CS3B3 ELISA Kt |
GA-E0451RT-96T |
GenAsia Biotech |
96T |
EUR 595.2 |
Mouse Chondroitin Sulfate Epitope 3B3(CS3B3 ELISA Kt |
QY-E20317 |
Qayee Biotechnology |
96T |
EUR 433.2 |
Rat Chondroitin Sulfate Epitope 3B3, CS3B3 ELISA Kt |
KLR0443 |
Krishgen |
1 x 96 wells |
EUR 341 |
Fish 11-keto Testosterone (11-KT) ELISA Kit |
NSL0002Fi |
Sunlong |
96 wells |
EUR 528 |
|
Canine 11-keto Testosterone(11-KT) ELISA Kit |
NSL1625Ca |
Sunlong |
1720 wells |
EUR 528 |
|
Bovine 11-keto Testosterone(11-KT) ELISA Kit |
NSL1911Bo |
Sunlong |
96 T |
EUR 528 |
|
Rat Chondroitin Sulfate Epitope 3B3,CS3B3 ELISA Kt |
YLA0979RA-48T |
Shanghai YL Biotech |
48T |
Ask for price |
Rat Chondroitin Sulfate Epitope 3B3,CS3B3 ELISA Kt |
YLA0979RA-96T |
Shanghai YL Biotech |
96T |
Ask for price |
M71-1000-483-WT-KT AF Systems Consumables |
142140 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-WT-KT AF Systems Consumables |
142141 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-1000-483-BK-KT AF Systems Consumables |
142142 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-BK-KT AF Systems Consumables |
142143 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-1000-483-YL-KT AF Systems Consumables |
142144 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-YL-KT AF Systems Consumables |
142145 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-1000-483-RD-KT AF Systems Consumables |
142146 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-RD-KT AF Systems Consumables |
142147 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-1000-483-BL-KT AF Systems Consumables |
142148 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: BMP71 ToughStripe-vloermarkeringstape met overlaminaat; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-BL-KT AF Systems Consumables |
142149 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-1000-483-GN-KT AF Systems Consumables |
142150 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: BMP71 ToughStripe-vloermarkeringstape met overlaminaat; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-GN-KT AF Systems Consumables |
142151 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-1000-483-OR-KT AF Systems Consumables |
142152 |
BRADY NV |
Kit of 1 Roll |
EUR 203.23 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
M71-2000-483-OR-KT AF Systems Consumables |
142153 |
BRADY NV |
Kit of 1 Roll |
EUR 304.84 |
Description: Description Dutch: ToughStripe-labels voor BMP71; Description French: Ruban de marquage au sol ToughStripe avec film de protection pour BMP71 |
Immortalized Human Bronchial Epithelial Cells (HBEC3-KT) - Cdk4/hTERT |
T0753 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 490 |