Assays
Rabbit Polyclonal Antibodies
in the Faculties we use rabbit and goat polyclonal antibodies.
Affinity purified through the isolation of specific polyclonal antibodies from antiserum by antigen affinity chromatography. This procedure exploits the specificity of the antibody-antigen interactions and typically yields >95% pure specific antibodies. Normally, the sera from host animals after immunization with cytokines, contain only small amounts (<5%) of cytokine-specific antibody which cannot be effectively isolated by standard purification procedures (e.g. ion exchange chromatography) or by non-antigen-specific affinity procedures, such as protein A/G affinity purification.
The large quantities of unrelated IgGs found in these inferior preparations can considerably increase the background when the antibody is used in analytical procedures such as ELISA, neutralization, immunohistochemistry, and Western Blotting. Therefore, the use of the superior antigen affinity-purified antibody preparations can help alleviate background in these analytical procedures.
Biotinylated Antibodies
The Faculties biotinylated antibodies are produced from highly pure and specific antigen affinity-purified polyclonal antibodies, and are therefore ideal for use in any analytical procedures that require biotinylated antibodies.
Monoclonal Antibodies
Mouse monoclonal clones made with full-length recombinant antigens and have been thoroughly screened for performance in a variety of applications.
ELISA Development Kits
ELISA Kits contain the necessary key components for the quantitative measurement of natural and/or recombinant proteins in a sandwich ELISA format. Each kit contains a capture antibody, a biotinylated detection antibody, a calibrated antigen standard, the avidin-peroxidase conjugate, and a detailed protocol.
QC-Testing:
Antibody Content
Verified by UV Spectroscopy and SDS-PAGE.
ELISA
Tested by “Enzyme Linked Immunosorbent Assay” (ELISA) for antibody-antigen detection and quantification of the antigen, using a solid-phase substrate such as a polystyrene plate, enzyme-coupled reagents, and additional detection materials. Extensive optimization and cross-reactivity testing for “ELISA Development Kit” (EDK) products.
Endotoxin Contamination
Tested with the kinetic LAL (Limulus Amebocyte Lysate) method to ensure the lowest endotoxin levels and the highest purity.
Immunhistochemistry (IHC) if applicable
Tested with an immuno-coloring assay (immunoenzymatic or immunofluorescent) for specific antibody-antigen recognition in a tissue or cell sample, using an enzyme-coupled reagent and other detection materials.
Neutralization if applicable
Tested to determine the antibody concentration required for a half-maximal inhibition [ND50] of the biological activity of the corresponding antigen.
Sterility
All products are sterile filtered through a 0.2μm filter.
Western Blot
Tested by an immunoblot assay for antibody-antigen detection and quantification, using SDS-PAGE, nitrocellulose membrane transfer, an enzyme-coupled reagent, and other detection materials.